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Bulletin of Pure and Applied Sciences-Botany (Started in 1982)
eISSN: 2320-3196
pISSN: 0970-4612
Impact Factor: 5.195 (2017)
DOI: 10.5958/2320-3196
Editor-in-Chief:  Prof. M.U. Charaya (Rtd),
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Article Details

Bulletin of Pure and Applied Sciences-Botany (Started in 1982)
Year : 2019, Volume & Issue : BPAS-Botany 38B(1), JAN-JUN 2019
Page No. : 18-22, Article Type : Original Aticle
Article DOI : 10.5958/2320-3196.2019.00003.X

Effect of Zeatin and Indole-3-Acetic Acid in Vitro Culture of Butea Buteiformis Voigt

Belai Meeta Suwal Singh
Author’s Affiliation : Central Department of Botany, Patan Multiple Campus Patan Lalitpur, Patan 44700, Nepal

Corresponding Author : Belai Meeta Suwal Singh Central Department of Botany, Patan Multiple Campus Patan Lalitpur, Patan 44700, Nepal.,
E-Mail:-[email protected]


Abstract

Butea buteiformis is a valuable tree with large trifoliate leaves with beautiful flower. The seeds were surface sterilized and cultured on half strength Murashige & Skoog (1962) (MS) medium. Nodal explants obtained from cultured were subcultured on different concentrations of zeatin (Zin) and Indole-3-acetic acid (IAA). The best proliferation of nodes and shoots were observed on the MS medium supplemented with 5 µM Zin and 1.0 M IAA. After 8 weeks of culture the propagated plants were acclimatized and transferred to the sand box containing 1:1 soil and sand. Well rooted plants were then established in the field. All the data collected were worked out statistically with SPSS, a system of analytical procedure. 

Keywords

Micropropagation, Murashige & Skoog (1962) medium, Nodal explants, sand rooting, Acclimatization.
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