A new series of Substituted-4-Biphenyl-acetamides have been synthesized by condensation of 4-Biphenyl acetic acid with different primary amines (aromatic and aliphatic). 4-Biphenyl Acetic Acid was first treated with Thionyl Chloride in dry benzene to prepare substituted-4-biphenyl acetyl chloride, which is then treated with different aliphatic or aromatic amines to synthesize various substituted-4-biphenyl Acid-amide derivatives. The Structure of newly synthesized compounds has been established by analytical and spectral methods. These all six biphenyl compounds were screened for antimicrobial activity. All compounds at different concentrations were analyzed for antifungal activity via disc diffusion method. The antimicrobial activity was screened against fungus Curvalaria lunata. All six compounds showed moderate to good activity against selected fungal strain. These synthesized compounds have shown Anti-fungal properties when they tested against Curvalaria lunata.