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Bulletin of Pure and Applied Sciences-Chemistry (Started in 1982)
eISSN: 2320-320X
pISSN: 0970-4620
Impact Factor: 6.991 (2018)
DOI: 10.5958/2320-320X
Editor-in-Chief:  Dr. Pruthviraj. R.D.
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Bulletin of Pure and Applied Sciences-Chemistry (Started in 1982)
Year : 2019, Volume & Issue : BPAS-Chemistry 38C(2), JUL-DEC 2019
Page No. : 150-157, Article Type : Original Aticle
Article DOI : 10.5958/2320-320X.2019.00016.5 (Received on 22.06.2019 Accepted on 26.08.2019)

Validated HPTLC method for simultaneous estimation of metoprolol succinate and ramipril in bulk drug and marketed formulation

Kamini Sethy1* Janhavi R. Rao2 K. Raja Rajeswari3 K.E.V. Nagoji4
Author’s Affiliation : 1,3,4Sri Venkateswara College of Pharmacy, Etcherla-532410, Srikakulum District, Andhra Pradesh, India. 2Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune-411038, Maharashtra, India.

Corresponding Author : Kamini Sethy, Sri Venkateswara College of Pharmacy, Etcherla-532410, Srikakulum District, Andhra Pradesh, India.,
E-Mail:-[email protected]


This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous   estimation of Metoprolol succinate and Ramipril as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of Methanol: Toluene: Ethyl Acetate: Ammonia (2.5:3:5:0.7v/v/v/v). Densitometric evaluation of the separated zones was performed at 209 nm. The two drugs were satisfactorily resolved with RF values 0.67, and 0.37 for Metoprolol Succinate and Ramipril, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (2000-12000 ng/spot for Metoprolol succinate and 200–1200 ng/spot for Ramipril), precision (intra-day RSD 0.471–1.036% and inter-day RSD 1.085–1.580% for Metoprolol Succinate and intra-day RSD 1.057–1.63% and inter-day RSD 1.024–1.746% for Ramipril), accuracy (98.95 ± 0.16 % for Metoprolol and 98.98 ± 0.41 % for Ramipril), and specificity, in accordance with ICH guidelines


Thin layer chromatography, densitometry, validation and quantification, Metoprolol Succinate, Ramipril.
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