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Bulletin of Pure and Applied Sciences-Chemistry (Started in 1982)
eISSN: 2320-320X
pISSN: 0970-4620
Impact Factor: 6.991 (2018)
DOI: 10.5958/2320-320X
Editor-in-Chief:  Dr. Pruthviraj. R.D.
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Bulletin of Pure and Applied Sciences-Chemistry (Started in 1982)
Year : 2019, Volume & Issue : BPAS-Chemistry 38C(2), JUL-DEC 2019
Page No. : 171-175, Article Type : Original Aticle
Article DOI : 10.5958/2320-320X.2019.00018.9 (Received on 24.09.2019 Accepted on 4.11.2019)

Iron Determination by Colorimetric Method Using O-Phenanthroline

Atri Deo Tripathi1,* K.A. Gupta2 Shally Malik3
Author’s Affiliation : 1,2,3Department of Chemistry, Faculty of Engineering, Teerthanker Mahaveer University, Moradabad, Uttar Pradesh 244001, India.

Corresponding Author : Atri D. Tripathi, Associate Professor, Dept. of Chemistry, College of Engineering, Teerthanker Mahaveer University, Moradabad, Uttar Pradesh 244001, India.,
E-Mail:-[email protected]


Fe2+ reaction with 1, 10-Phenanthroline determined the quantity of soluble iron (II) in the sample to transform a weakly colored iron into an intensely colored complex which could be used in the analysis. The substance absorbed certain wavelengths when a light from the source with a certain intensity and frequency range was passed to this intensely colored complex. The intensity of a solution's color is proportional to the absorbing species concentration and the absorption is proportional to the substance concentration. A separate concentration of standards was prepared and absorbance in 511 nm, the largest wavelength, was determined using Colorimeter. Using the same standard technique and reagents, a blank and three unknown samples were also prepared. A calibration curve was built following Beer's Law. The iron concentration was verified using the equation of the calibration curve and the absorption under the same experimental conditions of three unknowns.


Colorimeter, 1,10 phenanthroline, standard solutions, calibration, wavelength
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