Many elements of animal physiology, including as lifespan, general health, and reproductive success, have been studied, and cuticular hydrocarbon variation in Drosophila, are considered to be influenced by nutrition, which is one of the most significant environmental factors. A most important role of the insect CHC profile is to water-resistant the cuticle and avoid desiccation. Here we investigate the effect of male diet on cuticular hydrocarbon of Drosophila melanogaster, we found that carbohydrate and protein-rich diets have a significant impact on cuticular hydrocarbon variation in male D. melanogaster flies. Eighteen compounds were identified in male flies of D. melanogaster reared in all three different diets (normal media/ carbohydrate rich media/protein rich media), though, the concentration of CHC varies in the different diet significantly, which was suggest that male diet has a significant influence on cuticular hydrocarbon in D. melanogaster flies which inturn effect the attractiveness of the flies and fitness of Drosophila melanogaster.