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Bulletin of Pure and Applied Sciences-Zoology (Started in 1982)
pISSN: 0970-0765
Impact Factor: 5.395 (2017)
DOI: 10.5958/2320-3188
Editor-in-Chief:  Dr. Sushil Kumar Upadhyay
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Article Details

Bulletin of Pure and Applied Sciences-Zoology (Started in 1982)
Year : 2021, Volume & Issue : BPAS-Zoology 40A(2), JUL-DEC 2021
Page No. : 215-225, Article Type : Original Aticle
Article DOI : 10.5958/2320-3188.2021.00026.7 Article Info: Received on 13.05.2021 Accepted on 18.08.2021 Published on 15.12.2021

Assessment of 8-hydroxy-2′deoxyguanosine Activity and Histopathological Alteration in Liver and Intestine of Zebrafish (Daniorerio) Exposed to Arsenic Trioxide

Thakur Swati Singh1, Jain Abhishek2, Jain Roshni3, Jain Swati4, *Jain Subodh Kumar5
Author’s Affiliation : 1,2,3Department of Biotechnology, Dr Harisingh Gour University, Sagar, Madhya Pradesh 470003, India 4,5Department of Zoology, Dr Harisingh Gour University, Sagar, Madhya Pradesh 470003, India

Corresponding Author : Prof. Subodh Kumar Jain Department of Zoology, Dr Harisingh Gour University, Sagar, Madhya Pradesh 470003, India,
E-Mail:-[email protected]


The present study is aimed at assessing the chronic toxic effects of arsenic trioxide on liver and intestine of the zebrafish. For this purpose, histopathological changes in the liver and intestine tissues of adult zebrafish exposed to waterborne arsenic trioxide concentrations (50ppb and 500ppb respectively) for 90 days evaluated. Activation of 8-hydroxy-2′-deoxyguanosine (8-OHdG) was also assessed by immunofluorescence assay. We observed severe histopathological alterations, including cellular degeneration, vacuolization of hepatocytes, and an increase in the hepatic plate in the liver. Histopathological changes in the intestine includes vacuolization of the enterocytes, hyperplasia of goblet cells, displacement of the lamina propria, and disruption of the apical cytoplasm of epithelial cells that cover the intestinal villi were observed. Strong signs of immunofluorescence reaction for 8-OHdG in the exposed liver and intestine tissues in a dose-dependent manner has also been detected. Results of the study indicate that toxicity of arsenic trioxide lead to crucial histopathological alteration and induce oxidative DNA damage in both liver and intestine of zebrafish.


Arsenic Trioxide, Histopathology, Immunofluroscence, 8-OHdG, Zebrafish
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