Organophosphorous pesticides are mainly used to destroy harmful insects or pests which infect agricultural field, forests and houses. Increasing amount of agricultural, domestic and industrial chemicals into the aquatic environment lead to deleterious effects on the aquatic organisms. The biochemical study is recognized as a valuable tool for evaluating health status, physiological and pathological condition of fish. The present study deals with the level of imbalance evolved by the sub-lethal concentration of phorate on the tissue biomolecule of Channa punctatus. The pesticide, phorate was involved in the metabolic regulation of carbohydrate, protein and lipid in muscles, liver and kidney of Channa punctatus. Although, the mechanism is not clear that how phorate interferes in the metabolic process of these biomolecules. During recovery period, the tissue carbohydrate, protein and lipid content recorded a pattern towards normalcy when compared with 96 h exposed fishes.