PHARMACOGNOSTICAL, PHYTOCHEMICAL & PHARMACOLOGICAL EVALUATION OF BARK OF PLANT MIMUSOPS ELNGI: RESEARCH

Abstract

In Pharmacognostic study of bark of Mimusops elengi Linn. Macroscopic, microscopy, powder characteristic, physical parameters, and extractive values were studied. Microscopy of bark shows the presence of epidermis, covering trichomes, cholenchyma, and spongy parenchyma powder microscopy showed presence of starc grains, calcium oxalate crystals, and fibres. Bark  powder was subjected to soxhlet extraction with organic solvents of increasing polarity like petroleum ether, chloroform, methanol and water. Preliminary phytochemical screening of various extracts of bark of Mimusops elengi Linn shows the presence of steroids, alkaloids, glycosides, flavonoids, tannins, saponins and phenolic content. The methanolic extract shows valuable amount of Total Phenolic and Total Flavonoid content. Two phytoconstituent (Comp-A)  & amp; (Comp-B) was isolated from ethyl acetate soluble fraction of methanolic extract by column chromatography Both isolated compound were evaluated by qualitative and an attempt was made to characterize the isolated compound by U.V, IR, 1 H NMR and MASS spectroscopic studies. Charecterization revealed that comp-A may be flavonoid and comp-B may be tannins. The assessment of antioxidant activity was done by DPPH assay, Nitric oxide scavenging activity, and Reducing power determination. The Methanolic & aqueous extract shows significant antioxidant activity as compare to petroleum ether and chloroform extract. Ascorbic acid was used as standard for comparing results.

Anthelmintic activity of Methanol, petroleum ether, chloroform aqueous extracts & ethyl acetate soluble fraction of methanolic extracts of bark of Mimusops elengi Linn were investigated against Pheretima posthuma at Various concentrations (10,25,5050 mg/ml) of each extract were tested in the bioassay, which involved determination of time of paralysis and time of death of worms. Albendazole was included as standard reference and distilled water as control. The methanolic and ethyl acetate shows more potent activity.