Standardization of Various Parameters in the Basal Medium for the Growth of Bacillus subtilis Isolated from Soil Near to Ambala region

Abstract

To grow and proliferate a microorganism, an efficient medium that enriched with essential nutrients (carbon, nitrogen, minerals, growth factors, vitamins) is prior necessity for a microorganism. A media composition is a crucial part in the field of microbiology that can enhance or decline the bacterial growth. In this study we have isolated B. subtilis from wheat soil near to Ambala region. B. subtilis is an essential nitrogen fixing bacteria that provides accessible nitrogen in rhizosphere for the better growth of cereals. It improves the biological nitrogen fixation in soil using ecofriendly approach whereas artificial nitrogen approach is an energy demanding process that would be consuming approximately 2.0 % of the world's power by the end of this century. To improves the agricultural sustainability in cereals, it is necessary to optimize the growth of B. subtilis using different growth parameters. In the present study, 6 different media composition were chosen to evaluate the maximum proliferation of B. subtilis Moreover, different temperature and a pH range from 5 to 9 were selected to reveal the optimum growth and proliferation of B. subtilis. During this study, it was concluded that B. subtilis showed maximum growth with C1 medium composition (pH 7.0) after incubated at 30⁰ C for 16 hours shaking using UV Spectrophotometer. Furthermore, the optimized medium significantly promoted the growth of B. subtilis as compared to Burk’s media. Thus, the study would be beneficial for automation of B. subtilis for consortia preparation and hence improvement in agricultural sustainability.