Effect of Pottia lanceolata Linnaeus (Moss Extract) on Trichostrongylus in Sheep

Abstract

Bryophyte species, Pottia lanceolata was used as anthelmintic, against sheep nematode Trichostrongylus in vitro. Aqueous extract of Pottia lanceolata exhibited anthelmintic activity in dose dependent manner showing maximum efficacy at 20 mg/ml concentration. To test the efficacy of the treatment transcriptome analysis was done after isolating and extracting the mRNA. Gene expression pattern were studied in both the control and treated samples. A total of 1,763 genes were expressed in the control and treated samples, the genes expressed in control and treated samples differed. It was found that certain genes were up regulated in the control (257) and certain genes were down regulated (341). The transcriptome data as mRNA sequences were submitted to public database, GEO. The following are the ids of the submitted sequences. (GSE137609) Sheep Nematode Control (GSM4083097) Sheep Nematode treated (GSM4083098). The treatment was found to be quite effective, as expression of unique genes was observed, which seem to play a major role in apoptosis, cuticle damage, neuronal destruction, and triggering major signaling pathways, causing obliteration of worms. The bryophyte extract seems to be effective in inhibiting the nematode activity by impacting the genome and gradually leading to the extermination of the parasite and therefore may be suggested to be used as an effective nutraceutical for this helminth infection.