Anti-Inflammatory Activity of Root Assisted Synthesis of Copper Oxide Nanoparticle Using Gloriosa superba

Abstract

Introduction: The quest for natural, plant-based compounds in nanotechnology has heightened interest in medicinal plants with bioactive properties. Gloriosa superba, known as the flame lily, is valued in traditional medicine for its anti-inflammatory, analgesic, and antimicrobial effects, attributed to compounds like colchicine and gloriosine. This study evaluates G. superba root extracts as natural reducing agents for the green synthesis of copper oxide nanoparticles (CuO NPs) and investigates their anti-inflammatory properties.

Materials and Methods: G. superba roots were collected, washed, and air-dried before being ground into powder. Preparation of Gloriosa superba Root Extract and Synthesis of Copper Oxide Nanoparticles Done. Anti-inflammatory activity was assessed using the Egg Albumin (EA) assay and Membrane Stabilization assay, measuring the inhibition of inflammation at varying concentrations of CuO NPs (10, 25, and 50 µg/mL).

Results: The results indicated significant dose-dependent anti-inflammatory activity of G. superba-synthesized CuO NPs. All assays demonstrated increased inhibition with higher concentrations, achieving levels comparable to standard treatments.

Conclusion: This study highlights the potential of G. superba extracts for eco-friendly synthesis of CuO NPs with promising anti-inflammatory properties. These findings support the use of plant-based methods in developing effective therapeutic agents, warranting further exploration of their biomedical applications.