Study of the Wound healing effect of Nerium oleander and Ixora coccinea Leaves extract in albino Wistar rats by Excision Wound Healing Techniques

Abstract

Nerium oleander and Ixora coccinea are useful as medication in many abnormalities and diseases due to consisting of rutin, quercetin, kaempferol, and hesperidin. In this research, 50 percent hyrdo-methanolic extract of oleander leaves (HMEOL) and 50 percent hyrdo-methanolic extract of Ixora coccinea (HMEIL) were used to study the comparative evaluation for the wound healing activities for 16 days by the help of formulated ointments. Healthy Wistar rats were selected for this study. Phytochemical evaluation was helpful for the confirmation of Phytochemical chemical such as, Tannins, alkaloids, carbohydrate, glycosides, flavonoids, phenolic contents & protein. Three ointment such as 5 percent w/w HMEOL, 5 percent w/w HMEIL and 2.5 % w/w HMEOL + 2.5 % HMEIL were formulated for application of drug on animals’ skin of three different test groups. Study for Acute dermal toxicity of selected drugs was done by following guidelines acute dermal toxicity study 402. Comparative examination of Wound healing effect of drugs was done on the 0, 4^th, 8^th and 16^th day. Study for histopathological examination of tissues of different animals was done at the end of protocol of research. Formulated ointments of both drugs were found to be similar effective in treatment of open wound individually and their combined formulation found to be more effective as compare to individual.