Development of Photo-crosslinked Hydrogel incorporated with Curcumin/ Rutin for Wound Healing: An Invitro Study

Abstract

Introduction:

Wound healing is a complex biological process composed of interrelated and overlapping phases: inflammation, migration, proliferation, and maturation. In severe wounds, such as large skin injuries, second or third-degree burns, or diabetes foot ulcers, wound healing is a slow process and rarely leads to the complete restoration of tissue function. Hydrogels are three-dimensional polymeric networks that can swell in aqueous environment/biological fluids by absorbing the wound exudates while preventing wound dehydration.

Aim:

The aim of the study is to explore the development of a photo-crosslinked hydrogel incorporated with two potent natural compounds, curcumin and rutin and its efficacy in promoting wound healing.

Materials and methods:

Formation of hydrogel incorporated in correct proportions of curcumin and rutin was done. 10% gelatin was added and mixed with ethylene glycol to form gelatin methacrylate. 0.5 % of carrageenan was used. Rutin and curcumin in optimized concentration and 1:1 ratio stock sol. was used. It was then placed in 6 wells for room temp (30 minutes - 1 hour). Potassium chloride (KCl) was added for chemical cross linking, and it was then frozen at 80 degrees C and was lyophilized.

Results:

The results showed high ability of antimicrobial activity, Antioxidant activity, Compatibility and Anti-inflammatory properties for the photo-crosslinked hydrogel incorporated with curcumin and rutin.

Conclusion:

It can be concluded from our study that the development of a photo-crosslinked hydrogel incorporated with curcumin and rutin represents a promising approach for enhancing wound healing.

Keywords: Antimicrobial, anti-inflammatory, curcumin, nanoparticles, rutin, novel product, innovative technique, hydrogel, wound healing, anti-oxidant